CORTISOL,Total
Test:
137
CPT:
82533
Synonyms
Compound F
Hydrocortisone
Special Instructions
This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.
Expected Turnaround Time
Within 1 day
Specimen Requirements
Specimen
Within 1 day
Volume
1 mL
Minimum Volume
0.7 mL (Note: This volume does not allow for repeat testing.)
Container
Gel-barrier tube (preferred) or red-top tube
Collection
If a red-top tube is used, transfer separated serum to a plastic transport tube. Blood should be drawn at 8 AM and 4 PM to evaluate baseline diurnal variation (see Cortisol, AM & PM [104000]). Morning specimen is often ordered with ACTH level.
Storage Instructions
Room temperature
Causes for Rejection
Citrate plasma specimen; improper labeling
Test Details
Use
Establish the diagnosis of adrenocortical insufficiency, Addison’s disease, adrenocortical hypersecretion, Cushing’s syndrome. Malfunction of the organs in the hypothalamic-pituitary-adrenal cortex axis will result in alteration of the cortisol levels.